Eighteen fungal strains, with cellulolytic capabilities, were isolated from the microflora of Iran? All of the isolated fungal strains produced, under the preliminary conditions of growth and enzyme production, higher extracellular cellulolytic enzymes relative to Trichoderma reesei. For one of the isolated fungal strains, which turned out to belong to the Botrytis family and Trichoderma reesei optimized growth conditions were separately established. Maximum enzyme production for T.reesei, 400 mFPU/ml was achieved during 13 days of growth in a citrate/phosphate buffered media (pH=5) at 29?C? However? the isolated Botrytis sp. produced maximally 670 mFPU/ml by day 15th of growth at 29?C and in an unbuffered citrate system (pH=7)?
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