The chromosome number of Penaeus semisulcatus, which is
commonly known as the green tiger prawn and which lives in the
Persian Gulf, has been determined by three approaches and the
efficacies of these approaches have been compared. In one, various
tissues after intramuscular injection of colchicine, in another
embryonic cells and in the third blastema tissue regenerated after
amputation of a pair pereopods were used. All three approaches when
optimized were effective and their results suggest that the diploid
chromosome number of P. semisulcatus is 90. This figure falls within
the range of diploid chromosome numbers previously reported for
other species of shrimp (86 to 92). Most of the chromosomes of P.
semisulcatus are metacentric