Plant breeding improvement and using conventional methods isdifficult and relatively time consuming. Biotechnological methods,
especially tissue culture and cell suspension culture can be used to
transfer desirable traits such as salt resistance to forage plants such as
Medicago. Stem, leaf and root explants belonging to three Medicago
species: rigidula, scutellata and sativa, were initially cultured on MS
(Murashige and Skoog, 1962) medium containing 2 mg/L yeast
extract, 2mg/L 2,4-dichlorophenoxyacetic acid (2,4-D), 2 mg/L a-naphthaleneacetic
acid (NAA), 1.5 mg/L benzyladenine (BA), 0.5
mg/L Thiodizuron (TDZ) and 2 mg/L Kinetin. Only M. sativa
produced callus and was able to produce cell suspension in liquid
medium. Viable cells were then transferred to the same liquid medium
with 0, 30, 60, 90, 120 mM NaCl and finally, tolerant cell lines were
selected at 90 mM NaCl according to packed cell volume (PCV
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